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TCEP hydrochloride

Name: TCEP hydrochloride
Brand: TargetMol
SKU: T7087
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl) Copy Product Info

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Synonyms: Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl

Catalog No. T7087 Copy Product Info

Purity: 99.93%

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TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.

Cas No. 51805-45-9

Pack Size	Price	USA Stock	Global Stock
100 mg	$43	In Stock	In Stock
500 mg	$54	In Stock	In Stock
1 g	$68	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.93%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

TCEP hydrochloride AI Summary

TCEP hydrochloride shows no significant inhibitory activity against HDAC1 and HDAC6 enzymes. Additionally, it does not exhibit significant growth inhibition (GI50 > 50000.0 nM) on human A549, MCF7, and WI38 cells after 96 hours, as determined by the sulphorhodamine B assay..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a trialkylphosphine that selectively reduces proteolysis. TCEP hydrochloride is a non-thiol-reducing agent that promotes the binding of NF-κB-DNA.
Targets & IC50	MCF-7 cells:> 50 μM (GI50), WI-38 cells:> 50 μM (GI50), NS3/4A:12.8 μM, A549 cells:> 50 μM (GI50)
In vitro	The growth inhibitory activity (GI₅₀) of TCEP hydrochloride in A549, MCF-7 tumor cells and WI-38 normal cells was all greater than 50 μM.[1]
In vivo	TCEP hydrochloride can dose-dependently promote NF-κB-DNA binding within the concentration range of 0.25–6 mM, and can replace DTT for in vitro NF-κB binding experiments. Compared with DTT, TCEP hydrochloride does not significantly weaken the inhibitory effect of Hg^2+ on NF-κB-DNA binding. When 6 mM TCEP hydrochloride is present, 20 μM Hg^2+ can effectively block NF-κB-DNA binding, while at 0.25 mM DTT conditions, more than 100 μM Hg^2+ is required to achieve a similar effect. [2] TCEP hydrochloride can be used to reduce the disulfide bonds in hLF peptides. At a peptide concentration of 0.1 mM, adding 30-fold molar excess of TCEP hydrochloride and incubating at 37 ℃ for 2 hours can achieve effective reduction. [3]
Synonyms	Tris(2-carboxyethyl)phosphine hydrochloride, TCEP HCl

Chemical Properties

Molecular Weight	286.65
Formula	C₉H₁₆ClO₆P
Cas No.	51805-45-9
Smiles	Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O
Relative Density.	1.041 g/cm3 at 25℃

Storage & Solubility Information

Storage

Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (880.87 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 2.75 mg/mL (9.59 mM), Solution.

5% DMSO+95% Saline: 1.45 mg/mL (5.06 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4886 mL	17.4429 mL	34.8857 mL	174.4287 mL
5 mM	0.6977 mL	3.4886 mL	6.9771 mL	34.8857 mL
10 mM	0.3489 mL	1.7443 mL	3.4886 mL	17.4429 mL
20 mM	0.1744 mL	0.8721 mL	1.7443 mL	8.7214 mL
50 mM	0.0698 mL	0.3489 mL	0.6977 mL	3.4886 mL
100 mM	0.0349 mL	0.1744 mL	0.3489 mL	1.7443 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Tris(2-carboxyethyl)phosphine HydrochlorideTris(2-?carboxyethyl)?phosphineTCEP hydrochlorideTCEPInhibitorinhibit

Related Tags: TCEP hydrochloride chemical structure | TCEP hydrochloride in vivo | TCEP hydrochloride in vitro | TCEP hydrochloride formula | TCEP hydrochloride molecular weight